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In the latter case, the generation of new viral particles may be hampered directly, or mutated DNA may be properly packaged into new viral particles, but replication is subsequently halted in newly infected cells.

Tacrolimus trough blood levels were obtained once between days 16 and 18 and once between days 18 and 20, with at least 48 hr between the two measurements. Article information PLoS One. 2015; 10(8): e0133571.
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Therefore, we re-ran the analyses without this particular task.